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CAMK1D ELISA Kit (Human) (OKCA01138)

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96 Wells
$600.00
In Stock
Gene Symbol:
CAMK1D
Official Gene Full Name:
calcium/calmodulin dependent protein kinase ID
NCBI Gene Id:
57118
Alias Symbols:
RP11-462F15.1, CKLiK, CaM-K1, CaMKID, CaM kinase ID|CamKI-like protein kinase
Description of Target:
Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, activates CREB-dependent gene transcription, regulates calcium-mediated granulocyte function and respiratory burst and promotes basal dendritic growth of hippocampal neurons. In neutrophil cells, required for cytokine-induced proliferative responses and activation of the respiratory burst. Activates the transcription factor CREB1 in hippocampal neuron nuclei. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta.
Protein Name:
Calcium/calmodulin-dependent protein kinase type 1D
Swissprot Id:
Q8IU85
Protein Accession #:
NP_065130.1
Nucleotide Accession #:
NM_020397.3
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
9.8 pg/mL
Kit Range:
39 - 2,500 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01138 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01138 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology CAMK1D ELISA Kit (Human) (OKCA01138) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAMK1D has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAMK1D is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CAMK1D captured in the well.
Kit Component:
ComponentAmount
CAMK1D Microplate96 Wells (12 x 8 Well strips)
CAMK1D Lyophilized Standard2 x 2.5 ng
100X Biotinylated CAMK1D Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat10190-107
1:210094-105
1:49888-102
1:810495-107
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)8682-91
EDTA Plasma (n=4)9788-101
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CAMK1D.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CAMK1D.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
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