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C19orf10 ELISA Kit (Human) (OKEH01901)

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96 Wells
$450.00
In Stock
Application:
ELISA
Gene Symbol:
C19ORF10
Official Gene Full Name:
myeloid-derived growth factor
NCBI Gene Id:
56005
Alias Symbols:
EUROIMAGE1875335, IL25, IL-25, IL27, IL-27, IL27w, Interleukin-25, R33729_1, SF20, Stromal cell-derived growth factor SF20, UPF0556 protein C19orf10
Description of Target:
The protein encoded by this gene was previously thought to support proliferation of lymphoid cells and was considered an interleukin. However, this activity has not been reproducible and the function of this protein is currently unknown.
Protein Name:
Myeloid-derived growth factor
Swissprot Id:
Q969H8
Protein Accession #:
NP_061980.1
Nucleotide Accession #:
NM_019107.3
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 10 pg/mL
Kit Range:
15.6 - 1,000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 6.8% (n = 20)
Mean Inter-assay CV%: < 11.3% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology C19orf10 ELISA Kit (Human) (OKEH01901) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C19orf10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C19orf10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C19orf10 captured in well.
Kit Component:
ComponentAmount
C19orf10 Microplate96 Wells (12 x 8 Well strips)
C19orf10 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated C19orf10 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express C19ORF10.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express C19ORF10.
Datasheets / Downloads:
Printable datasheet for OKEH01901
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural human UPF0556 protein C19orf10. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
18 kDa
Protein Size (# AA):
173
Protocol:
Tips Information:
 
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