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Bradykinin ELISA Kit (Mouse) (OKCD02257)

96 Wells
In Stock
Gene Symbol:
Alias Symbols:
BRADYKININ; Kallidin I; L-Bradykinin; Callidin I; Kallidin 9
Description of Target:
BRADYKININ is a nonapeptide messenger that is enzymatically produced from KALLIDIN in the blood where it is a potent but short-lived agent of arteriolar dilation and increased capillary permeability. Bradykinin is also released from MAST CELLS during asthma attacks, from gut walls as a gastrointestinal vasodilator, from damaged tissues as a pain signal, and may be a neurotransmitter.
Protein Name:
Swissprot Id:
Species Reactivity:
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
0.48 pg/mL
Kit Range:
1.23 - 100 pg/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level target.
Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level target using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology BK ELISA Kit (Mouse) (OKCD02257) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-BK antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated BK and incubated. The BK found in the sample or standards competes with the biotinylated BK for limited binding sites on the immobilized anti-BK antibody. Wells are washed and Avidin-HRP conjugate is added, incubated then washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated BK captured in the well and inversely proportional to the amount of BK which was contained in the sample or standard.
Kit Component:
Anti-Bradykinin Microplate96 Wells (12 x 8 Well Strips)
Bradykinin Lyophilized Standard2
Bradykinin Biotin Complex1
100X Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Biotin Complex Diluent1 x 12 mL
Avidin/HRP Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Biotin Complex Reconstitution Buffer1 x 300 uL
Kit Linearity:
Matrix1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
EDTA plasma(n=5)99-105%96-103%93-101%96-104%
heparin plasma(n=5)92-101%80-98%97-105%94-105%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
EDTA plasma(n=5)88-9591
heparin plasma(n=5)91-10596
Kit Detection Method:
Colorimetric, OD450 nm
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Competitive Inhibition Immunoassay
Additional Information:
CAS: 58-82-2
PubChem: 439201
Tips Information:

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