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BPI ELISA Kit (Mouse) (OKCD02406)

96 Wells
$545.00
In Stock
Gene Symbol:
BPI
Official Gene Full Name:
bactericidal permeablility increasing protein
NCBI Gene Id:
329547
Alias Symbols:
9230105K17Rik, Bactericidal permeability-increasing protein, BPI
Description of Target:
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.
Protein Name:
Bactericidal permeability-increasing protein
Swissprot Id:
Q67E05
Protein Accession #:
NP_808518.1
Nucleotide Accession #:
NM_177850.3
Species Reactivity:
Mouse
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
13.7 pg/mL
Kit Range:
31.2-2,000 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Bpi was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Bpi was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10% (n=20)
Inter-Assay CV: <12% (n=24)
Kit Duration:
3 Hours
Kit Principle:
Aviva Systems Biology Bpi ELISA Kit (Mouse) (OKCD02406) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bpi has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wellss, incubated and removed.A biotinylated detector antibody specific for Bpi is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Bpi captured in well.
Kit Component:
ComponentAmount
Anti-Bpi Microplate96 Wells (12 x 8 Well strips)
Bpi Lyophilized Standard2
100X Biotinylated Bpi Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Kit Linearity:
Sample1:21:41:81:16
Serum (n=5)79-92%84-93%94-103%78-105%
EDTA Plasma (n=5)92-99%89-103%78-104%82-101%
Heparin Plasma (n=5)92-105%81-90%92-101%93-101%
Kit Recovery:
MatrixRecovery range (%)Average(%)
Serum (n=5)93-10196
EDTA Plasma (n=5)93-10597
Heparin Plasma(n=5)83-9787
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express BPI.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express BPI.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Bactericidal/Permeability Increasing Protein (BPI). No significant cross-reactivity or interference between Bactericidal/Permeability Increasing Protein (BPI) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Protein Size (# AA):
483
Protocol:
Tips Information:

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