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ATP4A ELISA Kit (Mouse) (OKCD00502)

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96 Wells
$555.00
In Stock
Application:
ELISA
Gene Symbol:
ATP4A
Official Gene Full Name:
ATPase, H+/K+ exchanging, gastric, alpha polypeptide
NCBI Gene Id:
11944
Alias Symbols:
Gastric H(+)/K(+) ATPase subunit alpha, H+/K+-ATPase alpha, H+K+-transporting alpha 1, Potassium-transporting ATPase alpha chain 1, Proton pump
Protein Name:
Sodium/potassium-transporting ATPase subunit alpha
Swissprot Id:
Q91WH7
Protein Accession #:
NP_061201
Nucleotide Accession #:
NM_018731.3
Species Reactivity:
Mouse
Sample Type:
Tissue homogenates and other biological fluids.
Sensitivity:
< 0.055 ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level Atp4a were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Atp4a were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology Atp4a ELISA Kit (Mouse) (OKCD00502) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp4a has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atp4a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Atp4acaptured in well.
Kit Component:
ComponentAmount
Anti-Atp4a Microplate96 Wells (12 x 8 Well Strips)
Atp4a Lyophilized Standard2 x 10 ng
100X Biotinylated Atp4a Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ATP4A.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ATP4A.
Datasheets/Manuals:
Printable datasheet/reference manual for OKCD00502
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay has high sensitivity and excellent specificity for detection of ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a). No significant cross-reactivity or interference between ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
1025
Protocol:
Tips Information:
 
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