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ATP1b1 ELISA Kit (Rat) (OKDD00805)

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96 Wells
$615.00
In Stock
Gene Symbol:
ATP1b1
Official Gene Full Name:
ATPase Na+/K+ transporting subunit beta 1
NCBI Gene Id:
25650
Alias Symbols:
Sodium/potassium-dependent ATPase subunit beta-1, Sodium/potassium-transporting ATPase subunit beta-1
Description of Target:
May regulate oxygen homeostasis in astrocytes, may play a role in cellular resistance to cardiac glycosides [rgd, feb 2006]
Protein Name:
sodium/potassium-transporting ATPase subunit beta-1
Swissprot Id:
P07340
Protein Accession #:
NP_037245.2
Nucleotide Accession #:
NM_013113.2
Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue homogenates
Sensitivity:
<0.057ng/mL
Kit Range:
0.156-10 ng/mL
Kit Reproducibility:

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

Kit Duration:
~4 Hours
Kit Principle:
Aviva Systems Biology ATP1b1 ELISA Kit (Rat) (OKDD00805) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for ATP1b1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ATP1b1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ATP1b1 captured in well.
Kit Component:
ComponentAmount
Anti-ATP1b1 Microplate96 Wells (12 x 8 Well Strips)
ATP1b1 Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ATP1b1 Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ATP1b1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ATP1b1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of ATP1b1. No significant cross-reactivity or interference between ATP1b1 and analogues was observed.
Assay Info:
Quantitative Sandwich ELISA
Molecular Weight:
35 kDa
Protein Size (# AA):
304
Protocol:
Tips Information:
 
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