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Aspergillus fumigatus IgG ELISA Kit (Human) (OKNA00103)

96 Wells
$285.00
In Stock
Alias Symbols:
AF
Description of Target:
Aspergillus fumigatus is a fungus of the genus Aspergillus, and is one of the most common Aspergillus species to cause disease in individuals with an immunodeficiency. A. fumigatus, a saprotroph widespread in nature, is typically found in soil and decaying organic matter. Colonies of the fungus produce thousands of small conidia (2-3 µm) that readily become airborne. The fungus is capable of growth at temperatures up to 50 °C, with conidia surviving at 70 °C. Humans are continuously in contact with these asexual spores and it is estimated that an individual inhales several hundred conidia each day; typically these are quickly eliminated by the immune system. Aspergillosis develops mainly in individuals who are immunocompromised, either from disease or from immunosuppressive drugs, and is a leading cause of death in acute leukemia and hematopoietic stem cell transplantation. The term aspergillosis comprises a number of different diseases caused by fungi of the genus Aspergillus. The most common forms are allergic bronchopulmonary aspergillosis (ABPA), pulmonary aspergilloma and invasive aspergillosis (IA). Allergic bronchopulmonary aspergillosis is characterized by an exaggerated response of the immune system to Aspergillus species. It occurs in patients suffering from asthma or cystic fibrosis. Clinically, ABPA manifests as a bronchial asthma with transient pulmonary infiltrates that may proceed to proximal bronchiectasis and lung fibrosis. Aspergilloma, commonly referred to as “fungus ball,” occurs in preexisting pulmonary cavities that were caused by tuberculosis, sarcoidosis, or other bullous lung disorders. The fungus settles in a cavity and grows until it forms a compact sphere, which incorporates dead tissue from the surrounding lung, mucus, and other debris. Patients are usually asymptomatic and often coexist for decades with aspergillomae prior to incidental diagnosis. However, it may cause hemoptysis. Invasive aspergillosis is a rapidly progressive, often fatal disease, targeting severely immunocompromised patients, including those with hematological malignancies such as leukemia, those who have received solid organ or hematopoietic stem cell transplants, and individuals with chronic granulomatous disease or advanced AIDS. IA is characterized by invasion of blood vessels, resulting in multifocal infiltrates. Dissemination to other organs, particularly the central nervous system, may occur.
Protein Name:
Aspergillus fumigatus IgG
Species Reactivity:
Human
Sample Type:
Serum, Plasma
Sensitivity:
Sensitivity is determined as the probability of the assay indicating a positive score in samples with the specific analyte present: > 98%
Kit Reproducibility:
MetricSample Typen=CV%
Intra-AssaySerum #1230.5530.032
Intra-AssaySerum #2241.6990.023
Intra-AssaySerum #3240.8270.073
Inter-AssaySerum #11217.550.043
Inter-AssaySerum #21213.720.056
Inter-AssaySerum #31237.080.049
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology Aspergillus fumigatus IgG ELISA Kit (Human) (OKNX00103) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Aspergillus fumigatus antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for Human IgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is qualitatively proportional to the amount of sample anti-Aspergillus fumigatus IgG captured the in well.
Kit Component:
ComponentAmount
Aspergillus fumigatus IgG Well Plate96 Wells (12 x 8 Well Strips)
IgG Sample Diluent1 x 100 mL
Stop Solution1 x 15 mL
20X Wash Solution1 x 50 mL
Anti-IgG HRP Conjugate1 x 20 mL
TMB Substrate1 x 15 mL
Aspergillus fumigatus Positive Control1 x 2 mL
Aspergillus fumigatus Cut-Off Control1 x 3 mL
Aspergillus fumigatus Negative Control1 x 2 mL
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
Specificity is determined as the probability of the assay indicating a negative score in samples absent of the specific analyte: > 95.7%
Assay Info:
Assay Methodology: Quantitative Reverse Capture Sandwich ELISA
Protocol:
Tips Information:

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