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APOB ELISA Kit (Pig) (OKCD01176)

96 Wells
$640.00
In Stock
Application:
ELISA
Gene Symbol:
APOB
NCBI Gene Id:
100725937
Alias Symbols:
Apo-B, FLDB, Apo B-100, Apo B-48
Protein Name:
Apolipoprotein B
Swissprot Id:
Q29433
Protein Accession #:
XP_003473207
Nucleotide Accession #:
XM_003473159.2
Species Reactivity:
Pig
Sample Type:
Serum, plasma and other biological fluids
Sensitivity:
< 2.82 ng/mL
Kit Range:
6.25-400 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level apoB were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level apoB were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
Kit Duration:
2.5 Hours
Kit Principle:
Aviva Systems Biology apoB ELISA Kit (Pig) (OKCD01176) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for apoB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for apoB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample apoBcaptured in well.
Kit Component:
ComponentAmount
Anti-apoB Microplate96 Wells (12 x 8 Well Strips)
apoB Lyophilized Standard2 x 400 ng
100X Biotinylated apoB Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Linearity:
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)81-98%95-104%94-101%80-92%
EDTA plasma(n=5)79-92%87-94%83-90%95-105%
heparin plasma(n=5)86-96%83-98%79-103%91-98%
Kit Recovery:
MatrixRecovery Range (%)Average (%)
serum(n=5)86-9590
EDTA plasma(n=5)92-103101
heparin plasma(n=5)88-10293
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express APOB.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express APOB.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of Apolipoprotein B (APOB). No significant cross-reactivity or interference between Apolipoprotein B (APOB) and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Protein Size (# AA):
1540
Protocol:
Tips Information:

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