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AP1G2 ELISA Kit (Human) (OKCA01105)

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96 Wells
$600.00
In Stock
Gene Symbol:
AP1G2
Official Gene Full Name:
adaptor related protein complex 1 gamma 2 subunit
NCBI Gene Id:
8906
Alias Symbols:
G2AD, OTTHUMP00000164600|clathrin-associated/assembly/adaptor protein, large, gamma-2|gamma2-adaptin
Description of Target:
May function in protein sorting in late endosomes or multivesucular bodies (MVBs). Involved in MVB-assisted maturation of hepatitis B virus (HBV).
Protein Name:
AP-1 complex subunit gamma-like 2
Swissprot Id:
O75843
Protein Accession #:
NP_003908.1
Nucleotide Accession #:
NM_003917.4
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity:
5.8 pg/mL
Kit Range:
23.5 - 1,500 pg/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01105 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01105 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology AP1G2 ELISA Kit (Human) (OKCA01105) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AP1G2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AP1G2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AP1G2 captured in the well.
Kit Component:
ComponentAmount
AP1G2 Microplate96 Wells (12 x 8 Well strips)
AP1G2 Lyophilized Standard2 x 1.5 ng
100X Biotinylated AP1G2 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat8584-91
1:29691-102
1:48481-87
1:8108103-114
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)9287-96
EDTA Plasma (n=4)10395-107
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express AP1G2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express AP1G2.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
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