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Anti-ROCKII Antibody (Paired Thr396), Non-Phospho (OAAI00621)

100 ug
$305.00
In Stock
Gene Symbol:
ROCK2
NCBI Gene Id:
9475
Official Gene Full Name:
Rho-associated, coiled-coil containing protein kinase 2
Swissprot Id:
O75116
Alias Symbols:
ROCK-II,
Replacement Item:
This antibody may replace item sc-100425 from Santa Cruz Biotechnology.
Description of Target:
Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins.
ROCK-II induces a depression in maximum ATPase Rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
Molecular Weight:
165 kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Application:
ELISA, WB, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ROCK2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ROCK2.
Immunogen:
A synthetic peptide corresponding to the epitope -VETFP- without a phosphorylation site Thr396 of Human RockII protein.
This sequence is identical among Human, Mouse, Rat, chicken, dog and bovine.
Species Reactivity:
Human, Mouse, Rat, Chicken, Bovine, Dog
Reconstitution and Storage:
The antibodies are stable for 12 months from date of receipt when stored at -20C to -70C. The antibodies can be stored at 2C to 8C for three month without detectable loss of activity.
Avoid repeated freezing thawing cycles.
Datasheets/Manuals:
Printable datasheet for Rabbit Anti-ROCKII Antibody (Paired Thr396), Non-PhosphoOAAI00621
Specificity:
This antibody recognizes ~165 kDa of Human ROCKII without a phosphorylation site ofThr396. It does not react to phosphorylated protein. This antibody also reacts with Mouse and Rat. The other species are not tested.
Isotype:
Rabbit IgG
Application Info:
Positive Control: 3T3 Cell Lysate
Western Blot: 0.1-1 μg/ml
ELISA: 0.01-0.1 μg/ml
IP: 2-5 μg/ml
IHC: 0.5-2 μg/ml
Target Reference:
Susan Vahebi, et al, Functional Effects of Rho-Kinase–Dependent Phosphorylation of Specific Sites on Cardiac Troponin.
Circulation Research. 2005;96:740-747.
Protocol:
Tips Information:

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