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ADORA2A ELISA Kit (Mouse) (OKEH03614)

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96 Wells
$485.00
In Stock
Gene Symbol:
ADORA2A
Official Gene Full Name:
adenosine A2a receptor
NCBI Gene Id:
11540
Alias Symbols:
A2a, Rs, A2AAR, A2aR, AA2AR, Adenosine receptor A2a, MGC118414
Description of Target:
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Protein Name:
Adenosine receptor A2a
Swissprot Id:
Q60613
Protein Accession #:
NP_033760.2
Nucleotide Accession #:
NM_009630.3
Species Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
0.156 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: <= 8.3% (n = 20)
Mean Inter-assay CV%: <= 10.4% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology ADORA2A ELISA Kit (Mouse) (OKEH03614) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adora2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Adora2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adora2a captured in well.
Kit Component:
ComponentAmount
Adora2a Microplate96 Wells (12 x 8 Well strips)
Adora2a Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Adora2a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Adora2a.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Adora2a.
Datasheets / Downloads:
Printable datasheet for OKEH03614
Reconstitution and Storage:
Store at 2 - 8C. Do not use the kit beyond the expiration date.
Specificity:
This assay recognizes recombinant and natural mouse Adenosine receptor A2a. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
45 kDa
Protein Size (# AA):
410
Protocol:
Tips Information:
 
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