Now Offering Over 102,157 Antibodies & 44,722 Antigens!

ADAMTSL4 ELISA Kit (Human) (OKCA01095)

Scroll Horizontally to view all Images
Print Page
96 Wells
$600.00
In Stock
Gene Symbol:
ADAMTSL4
Official Gene Full Name:
ADAMTS like 4
NCBI Gene Id:
54507
Alias Symbols:
TSRC1, OTTHUMP00000032789|thrombospondin repeat containing 1
Description of Target:
Positive regulation of apoptosis. May facilitate FBN1 microfibril biogenesis.
Protein Name:
ADAMTS-like protein 4
Swissprot Id:
Q6UY14
Protein Accession #:
NP_001275536.1
Nucleotide Accession #:
NM_001288607.1
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates
Sensitivity:
0.039 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Intra-assay Precision: 3 samples with known low, middle and high level OKCA01095 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level OKCA01095 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
Kit Duration:
~ 3.5 Hours
Kit Principle:
Aviva Systems Biology ADAMTSL4 ELISA Kit (Human) (OKCA01095) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ADAMTSL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ADAMTSL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ADAMTSL4 captured in the well.
Kit Component:
ComponentAmount
ADAMTSL4 Microplate96 Wells (12 x 8 Well strips)
ADAMTSL4 Lyophilized Standard2 x 10 ng
100X Biotinylated ADAMTSL4 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Kit Linearity:
DilutionMean Recovery (%)Range (%)
Neat9287-96
1:210198-104
1:48580-90
1:810298-106
Kit Recovery:
SampleMean Recovery (%)Range (%)
Serum (n=5)8481-87
EDTA Plasma (n=4)9693-99
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ADAMTSL4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ADAMTSL4.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sanadwich ELISA
Protocol:
Tips Information:
 
X
Ask a Question

Tell us what you think about this item!

Write A Review
    Please, wait...