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ACAN ELISA Kit (Dog) (OKEH04621)

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96 Wells
$796.00
In Stock
Gene Symbol:
ACAN
NCBI Gene Id:
403828
Alias Symbols:
AGC1, CSPCP
Description of Target:
This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
Protein Name:
Aggrecan core protein
Swissprot Id:
Q28343
Protein Accession #:
NP_001106926.2
Nucleotide Accession #:
NM_001113455.2
Species Reactivity:
Dog
Sample Type:
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.312 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Reproducibility:
Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology ACAN ELISA Kit (Dog) (OKEH04621) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACAN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for ACAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACAN captured in well.
Kit Component:
ComponentAmount
Anti-ACAN Microplate96 Wells (12 x 8 Well Strips)
ACAN Lyophilized Standard2
Sample Diluent1 x 20 mL
ACAN Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ACAN.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ACAN.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural dog Aggrecan core protein. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
241 KDa
Protein Size (# AA):
2333
Protocol:
Tips Information:
 
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