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Aviva's Custom Services - Rabbit Polyclonal Services

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 PolyclonalMonoclonal Peptide 
  Rabbit Polyclonal Antibody Services Mouse Monoclonal Antibody Services Peptide Services

Polyclonal antibodies are a mixture of antibodies specific for a set of epitopes on a target protein/peptide.  Aviva Systems Biology has been producing quality rabbit polyclonal antibodies to peptides and proteins for over 10 years.

Aviva Systems Biology offers a production protocol that combines antibody production expertise, a quick turnaround time and extremely affordable pricing. Aviva can help you with your specific project. Learn more about our custom antibody packages by contacting us at  info@avivasysbio.com.

An overview of our standard rabbit polyclonal production packages
are as follows:

Download Aviva's Custom Rabbit Polyclonal Antibody Service Packages PDF (290KB)

Please note for all packages, the ELISA titer is sent to researcher before final bleed.

 Package NumberDescriptionPackage ContentsTimePrice
  Polyclonal package 1
(From protein to antiserum)
  • Customer provides antigen protein
  • Protein will be checked by SDS-PAGE
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • We guarantee the ELISA titer of 1:32,000 or better.
  • 1-2ml pre-bleed
  • 40-60ml post-bleed
  • Project datasheet including ELISA data
10-12 weeks $800.00
  Polyclonal package 2
(From protein to antigen purified antibody)
  • Customer provides antigen protein
  • Protein will be checked by SDS-PAGE
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • Dot blot confirmation
  • We guarantee the ELISA titer of 1:32,000 or better, and dot blot is positive at antibody dilution 1:20,000, antigen peptide 100 ng.
  • 1-2ml pre-bleed
  • 2-5mg affinity purified antibody
  • Project datasheet including ELISA and dot blot data
10-12 weeks $1,050.00
  Polyclonal package 3
(From peptide design to antiserum)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • We guarantee the ELISA titer of 1:20,000 or better.
  • 2-3mg antigen peptide
  • 1-2ml pre-bleed
  • 40-60ml post-bleed
  • Project datasheet including ELISA data
12-15 weeks $1,200.00
  Polyclonal package 4
(From peptide design  to antigen purified antibodies)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • Dot blot confirmation
  • We guarantee the ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, antigen peptide 100 ng.
  • 2-3mg antigen peptide
  • 1-2ml pre-bleed
  • 2-5mg affinity purified antibody
  • Project datasheet including ELISA and dot blot data
12-15 weeks $1,425.00
  Polyclonal package 5
(From plasmid  to antiserum)
  • Customer provides expression vector
  • Express and purify the antigen protein
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • We guarantee the ELISA titer of 1:32,000 or better.
  • 1mg antigen protein
  • 1-2ml pre-bleed
  • 40-60ml post-bleed
  • Project datasheet including ELISA data
12-15 weeks $1,500.00
  Polyclonal package 6
(From plasmid  to antigen purified antibody)
  • Customer provides expression vector
  • Express and purify the antigen protein
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • Dot blot confirmation
  • We guarantee the ELISA titer of 1:32,000 or better, and dot blot is positive at antibody dilution 1:20,000, antigen peptide 100 ng.
  • 1mg antigen protein
  • 1-2ml pre-bleed
  • 2-5mg affinity purified antibody
  • Project datasheet including ELISA and dot blot data
12-15 weeks $1,750.00
  Polyclonal package 7
(From cDNA  to antiserum)
  • Customer provides protein information
  • We clone the gene and construct the expression vector for fusion protein
  • Express and purify the antigen protein
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • We guarantee the ELISA titer of 1:32,000 or better.
  • Recombinant vector
  • 1mg protein
  • 1-2ml pre-bleed
  • 40-60ml post-bleed
  • Project datasheet including ELISA data
14-16 weeks $2,350.00
  Polyclonal package 8
(From cDNA to antigen purified antibody)
  • Customer provides gene and protein information
  • We clone the gene and construct the expression vector for fusion protein
  • Express and purify the antigen protein
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • Dot blot confirmation
  • We guarantee the ELISA titer of 1:32,000 or better, and dot blot is positive at antibody dilution 1:20,000, antigen peptide 100 ng.
  • Recombinant vector
  • 1mg protein
  • 1-2ml pre-bleed
  • 2-5mg affinity purified antibody
  • Project datasheet including ELISA and dot blot data
14-16 weeks $2,600.00
  Polyclonal package 9
(Phospho-specific polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide/EA (non- & phosphor- peptide)
  • 1-2ml pre-bleed
  • 2-5mg antibody against unphospho-peptide
  • 2-5mg phospho-specific antibody
  • Project datasheet including ELISA and dot blot data
13-16 weeks $2,700.00
  Polyclonal package 10
(Lys mono-methylated specific polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide/EA (non- & mono-methylated peptide)
  • 1-2ml pre-bleed
  • 2-5mg antibody against unmethylated peptide
  • 2-5mg Lys(Me) specific antibody
  • Project datasheet including ELISA and dot blot data
13-16 weeks $2,700.00
  Polyclonal package 11  
(Lys dimethylated specific polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide/EA (non- & di-methylated peptide)
  • 1-2ml pre-bleed
  • 2-5mg antibody against unmethylated peptide
  • 2-5mg Lys(Me2) specific antibody
  • Project datasheet including ELISA and dot blot data
13-16 weeks $2,700.00
  Polyclonal package 12
(Lys trimethylated specific polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide/EA (non- & tri-methylated peptide)
  • 1-2ml pre-bleed
  • 2-5mg antibody against unmethylated peptide
  • 2-5mg Lys(Me3) specific antibody
  • Project datasheet including ELISA and dot blot data
13-16 weeks $2,700.00
  Polyclonal package 13 
(Arg methylation-specific polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide/EA (non- & methylated peptide)
  • 1-2ml pre-bleed
  • 2-5mg antibody against unmethylated peptide
  • 2-5mg Arg(Me) specific antibody
  • Project datasheet including ELISA and dot blot data
13-16 weeks $2,700.00
  Polyclonal package 14
(Arg di-methylation (Symmetry)-specific polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide/EA (non- & dimethylated peptide)
  • 1-2ml pre-bleed
  • 2-5mg antibody against unmethylated peptide
  • 2-5mg Arg(Me2) specific antibody
  • Project datasheet including ELISA and dot blot data
13-16 weeks $2,700.00
  Polyclonal package15
(Arg di-methylation (Asymmetric)-specific polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide/EA (non- & dimethylated peptide)
  • 1-2ml pre-bleed
  • 2-5mg antibody against unmethylated peptide
  • 2-5mg Arg(Me2) specific antibody
  • Project datasheet including ELISA and dot blot data
13-16 weeks $2,700.00
  Polyclonal package 16
(Western guaranteed polyclonal antibody)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize two New Zealand rabbits
  • Titer the immunization by ELISA
  • Post-bleed collection
  • Antigen affinity purification
  • We guarantee the ELISA titer of 1:20,000 or better, and the antibody is suitable for Western blot.
  • 2-3mg antigen peptide
  • 1-2ml pre-bleed
  • 2-5mg affinity purified antibody
  • Project datasheet including ELISA and Western blot data
15-18 weeks $3,000.00

Peptide Services

 Service ItemsTimePrice
  Peptide design (suggestion) 1-2 days Free
  Peptide synthesis 2 weeks Inquire
  Peptide conjugation (KLH/BSA/OVA,≤5mg) 2-3 days $245

Protein Services

 Service ItemsTimePrice
  Gene synthesis 3-4 weeks $2.52/aa
  Gene amplification (Template provided by customer,≤1000bp) 2-3 weeks $308
  Vector construction 2-3 weeks $616
  Recombinant selection 1 week $308
  Protein expression and purification 2-3 weeks $616

Polyclonal Antibody Services

 Service ItemsTimePrice
  Protein checked by SDS-PAGE 2-3 days $91
  Pre-immune serum collection 1 day $14
  Immunization & serum collection (2 rabbits) 10 weeks $805
  Immunization & serum collection (2 rabbits, Fast) 6-8 weeks $924
  ELISA identification 2-3 days $63
  Prescreen (ELISA) 2-3 days $63
  Prescreen (Western Blot) 1 week $154
  Prescreen (Dot Blot) 1 week $63
  Additional rabbit (1 rabbit) 6-10 weeks $308
  Animal maintenance (1 rabbit) 1 week $31
  Additional bleed (10ml, 1 rabbit) 1 week $47
  Extension boost and bleed (10ml, 1 rabbit) 1 week $63
  Immunization & serum collection (1 goat) 20 weeks $1575
  Immunization & serum collection (2 rats) 10 weeks $700
  Immunization & serum collection (4 mice) 10 weeks $805
  Immunization & serum collection (2 guinea pigs) 11 weeks $910
       

Antibody Purification Services

 Service ItemsTimePrice
  Saturated ammonium sulfate precipitation (≤60ml) 3-5days $126
  Protein A/G affinity purification (≤60ml) 3-5 days $182
  Antigen affinity column preparation 2-3 days $245
  Antigen affinity purification (≤60ml) 3-5 days $308
       

Antibody Validation Services

 Service ItemsTimePrice
  ELISA identification (1-10 samples) 2-3 days $63
  Cell extraction (1 sample) 2-3 days $31
  Tissue extraction (1 sample) 2-3 days $31
  Protein concentration assay (1 sample) 2-3 days $14
  Western Blot identification (1-8 samples) 1 week $245
  Dot Blot identification (1-10 samples) 2-3 days $62
  IHC identification (section provided by customer,1 photo) 1 week $31

Antibody Labeling Services

 Service ItemsTimePrice
  HRP labeling (≤5mg) 1-2 weeks $308
  Gene amplification (Template provided by customer,≤1000bp) 1-2 weeks $560