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Aviva's Custom Services - Mouse Monoclonal Services

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 PolyclonalMonoclonal Peptide 
  Rabbit Polyclonal Antibody Services Mouse Monoclonal Antibody Services Peptide Services

Monoclonal antibodies are produced from an immortalized B-cell (hybridoma) isolated from the spleen of an antigen immunized mouse.  Monoclonal antibodies recognize only a single epitope.  Once a single, stable hybridoma clone is isolated, the antibodies can be made either in vitro using cell culture techniques, or in vivo from ascites. Aviva can help you with your specific project. Learn more about our custom antibody packages by contacting us at  info@avivasysbio.com.

Aviva offers monoclonal antibody services as well as a variety of validation services.

An overview of our standard mouse monoclonal production packages are as follows:

Download Aviva's Custom Mouse Monoclonal Antibody Service Packages PDF (254KB)

 Package Number DescriptionPackage ContentsTimePrice
  Monoclonal package 1
(From protein to ascites purification)
  • Customer provides antigen protein
  • Protein will be checked by SDS-PAGE
  • Immunize four mice (Balb/C)
  • Titer the immunization by ELISA
  • Hybridoma fusion and cloning
  • Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones)
  • IgG isotype determination
  • Ascites are prepared from two hybridoma clones which customer selects
  • Antigen affinity purification
  • Dot blot for QC
  • We guarantee the ELISA titer of 1:20,000 or better.
  • 50ul pre-bleed (1:10dilution)
  • 2-5 hybridoma clones
  • 2-5mg purified antibody from two hybridoma clones
  • Project datasheet including ELISA,  dot blot and isotype determination data
18-24 weeks $6,175.00
  Monoclonal package 2
(From peptide design  to ascites purification)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize four mice (Balb/C)
  • Titer the immunization by ELISA
  • Hybridoma fusion and cloning
  • Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones)
  • IgG isotype determination
  • Ascites are prepared from two hybridoma clones which customer selects
  • Antigen affinity purification
  • Dot blot for QC
  • We guarantee the ELISA titer of 1:20,000 or better.
  • 2-3mg antigen peptide
  • 50ul pre-bleed (1:10dilution)
  • 2-5 hybridoma clones
  • 2-5mg purified antibody from two hybridoma clones
  • Project datasheet including ELISA,  dot blot and isotype determination data
20-26 weeks $6,790.00
  Monoclonal package 3
(From plasmid to ascites purification)
  • Customer provides expression vector
  • Express and purify the antigen protein
  • Immunize four mice (Balb/C)
  • Titer the immunization by ELISA
  • Hybridoma fusion and cloning
  • Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones)
  • IgG isotype determination
  • Ascites are prepared from two hybridoma clones which customer selects
  • Antigen affinity purification
  • Dot blot for QC
  • We guarantee the ELISA titer of 1:20,000 or better.
  • 1mg protein
  • 50ul pre-bleed (1:10dilution)
  • 2-5 hybridoma clones
  • 2-5mg purified antibody from two hybridoma clones
  • Project datasheet including ELISA,  dot blot and isotype determination data
20-26 weeks $7,100.00
  Monoclonal package 4
(From cDNA to ascites purification)
  • Customer provides gene and protein information
  • We clone the gene and construct the expression vector for fusion protein
  • Express and purify the antigen protein
  • Immunize four mice (Balb/C)
  • Titer the immunization by ELISA
  • Hybridoma fusion and cloning
  • Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones)
  • IgG isotype determination
  • Ascites are prepared from two hybridoma clones which customer selects
  • Antigen affinity purification
  • Dot blot for QC
  • We guarantee the ELISA titer of 1:20,000 or better.
  • recombinant vector
  • 1mg protein
  • 50ul pre-bleed (1:10dilution)
  • 2-5 hybridoma clones
  • 2-5mg purified antibody from two hybridoma clones
  • Project datasheet including ELISA,  dot blot and isotype determination data
22-26 weeks $7,700.00
  Monoclonal package 5
(Phospho- specific monoclonal antibodies)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize six mice (Balb/C)
  • Titer the immunization by ELISA
  • Hybridoma fusion and cloning
  • Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones)
  • IgG isotype determination
  • Ascites are prepared from two hybridoma clones which customer selects
  • Antigen affinity purification
  • Dot blot for QC
  • We guarantee ELISA titer of 1:20,000 or better, and dot blot is positive at antibody dilution 1:20,000, phospho-peptide 100 ng, but negative at antibody dilution 1:100, unphospho-peptide 100ng.
  • 2-3mg peptide including non- and phosphor-peptide
  • 50ul pre-bleed (1:10dilution)
  • 2-5 hybridoma clones specific for phosphor-peptide
  • 2 hybridoma clones for non-phospho-peptide
  • 2-5mg purified phosphor-specific antibody from two hybridoma clones
  • 2-5mg purified non-specific antibody from two hybridoma clones
  • Project datasheet including ELISA,  dot blot and isotype determination data
21-26 weeks $8,650.00
  Monoclonal package 6
(Western guaranteed monoclonal antibodies)
  • Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer.  Customer will make his final decision)
  • Synthesize one peptide (12-22aa, purity>85%)
  • Conjugate the peptide to carrier protein (KLH, BSA or OVA)
  • Immunize six mice (Balb/C)
  • Titer the immunization by ELISA
  • Hybridoma fusion and cloning
  • Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). 
  • IgG isotype determination
  • Ascites are prepared from two hybridoma clones which customer selects
  • Antigen affinity purification
  • Western blot for QC
  • We guarantee the ELISA titer of 1:20,000 or better, and the antibody is suitable for Western blot.
  • 2-3mg peptides
  • 50ul pre-bleed (1:10dilution)
  • 2-5 hybridoma clones
  • 2-5mg purified antibody from two hybridoma clones
  • Project datasheet including ELISA, Western blot and isotype determination data
20-26 weeks $11,100.00
           

Peptide Services

 Service ItemsTimePrice
  Peptide design (suggestion) 1-2 days Free
  Peptide synthesis 2 weeks Inquire
  Peptide conjugation (KLH/BSA/OVA,≤5mg) 2-3 days $245

Protein Services

 Service ItemsTimePrice
  Gene synthesis 3-4 weeks $2.52/aa
  Gene amplification (Template provided by customer,≤1000bp) 2-3 weeks $308
  Vector construction 2-3 weeks $616
  Recombinant selection 1 week $308
  Protein expression and purification 2-3 weeks $616

Monoclonal Antibody Services

 Service ItemsTimePrice
  Protein checked by SDS-PAGE 2-3 days $91
  Pre-immune serum collection 1 day $14
  Immunization & serum collection (4 mice) 6-10 weeks $805
  ELISA identification 2-3 days $62
  Fusion and hybridoma development 10-16 weeks $4942
  Isotype determination 1 week $154
  Ascites production (3 mice) 3-4 weeks $462
  Roller bottle cell culture (1L) 5-7 weeks $616
  Prescreen (ELISA) 2-3 days $63
  Prescreen (Western Blot) 1 week $154
  Prescreen (Dot Blot) 1 week $63
  Clone maintenance (1 clone) 1 week $21
  Clone storage (1 clone) 1 week $14

Antibody Purification Services

 Service ItemsTimePrice
  Saturated ammonium sulfate precipitation (≤60ml) 3-5 days $126
  Protein A/G affinity purification (≤60ml) 3-5 days $182
  Antigen affinity column preparation 2-3 days $245
  Antigen affinity purification (≤60ml) 3-5 days $308
       

Antibody Validation Services

 Service ItemsTimePrice
  ELISA identification (1-10 samples) 2-3 days $63
  Cell extraction (1 sample) 2-3 days $31
  Tissue extraction (1 sample) 2-3 days $31
  Protein concentration assay (1 sample) 2-3 days $14
  Western Blot identification (1-8 samples) 1 week $245
  Dot Blot identification (1-10 samples) 2-3 days $62
  IHC identification (section provided by customer,1 photo) 1 week $31

Antibody Labeling Services

 Service ItemsTimePrice
  HRP labeling (≤5mg) 1-2 weeks $308
  Gene amplification (Template provided by customer,≤1000bp) 1-2 weeks $560