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8ISOPG ELISA Kit (Human) (OKEH00590)

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96 Wells
$420.00
In Stock
Application:
ELISA
Gene Symbol:
8ISOPG
Official Gene Full Name:
8-iso-prostaglandin
Alias Symbols:
8-iso-prostaglandin
Protein Name:
8ISOPG
Species Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 10 pg/mL
Kit Range:
15.6 - 1,000 pg/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology 8ISOPG ELISA Kit (Human) (OKEH00590) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human 8ISOPG antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated 8ISOPG and incubated. The 8ISOPG found in the sample or standards competes with the biotinylated 8ISOPG for limited binding sites on the immobilized anti-human 8ISOPG antibody. Excess unbound biotinylated 8ISOPG and sample or standard 8ISOPG is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated 8ISOPG captured in the well and inversely proportional to the amount of 8ISOPG which was contained in the sample or standard.
Kit Component:
ComponentAmount
Anti-8ISOPG Microplate96 Wells (12 x 8 Well strips)
8ISOPG Lyophilized Standard2
Sample Diluent1 x 20 mL
100X 8ISOPG-Biotin Complex1 x 60 µL
100X Avidin-HRP Conjugate1 x 120 µL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural Human 8-iso-Prostaglandin F2α. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Competitive ELISA
Protocol:
Tips Information:
 
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