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1,25-dihydroxyvitamin D3 ELISA Kit (Rat) (OKCA00192)

96 Wells
$480.00
In Stock
Gene Symbol:
1,25 DHVD3
Alias Symbols:
Calcitriol, 1,25 DHVD3, 1,25-dihydroxyvitamin D3
Description of Target:
1,25-Dihydroxyvitamin D3 is the physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.
Protein Name:
1,25-Dihydroxy vitamin D3
Species Reactivity:
Rat
Sample Type:
Serum
Sensitivity:
< 250 fmoL/L
Kit Range:
1,000 fmol/L - 5,000 fmol/L
Kit Reproducibility:
Mean Intra-Assay Precision %CVMean Inter-Assay Precision %CV
Sample123123
n202020202020
Mean (fmolL)2001.252016.371977.661986.221999.12009.88
SD0.0730.0780.0620.0880.1020.091
CV%5.1645.4974.3156.1387.1556.397
Kit Duration:
< 4 Hours
Kit Principle:
Aviva Systems Biology 1,25-dihydroxyvitamin D3 ELISA Kit (Rat) (OKCA00192) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Rat 1,25-dihydroxyvitamin D3 and blocked. Standards or samples are added to the plate wells along with a horseradish peroxidase (HRP) conjugated antibody specific to 1,25-dihydroxyvitamin D3 and incubated. 1,25-dihydroxyvitamin D3 immobilized in the wells competes with the liquid phase 1,25-dihydroxyvitamin D3 contained in the samples or standards for binding with the HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody. Wells are washed removing unbound 1,25-dihydroxyvitamin D3, unbound HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody or 1,25-dihydroxyvitamin D3/HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody complex. HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody bound to 1,25-dihydroxyvitamin D3 immobilized in the well remains. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of HRP-Conjugated 1,25-dihydroxyvitamin D3 Antibody captured in the well and inversely proportional to the amount of 1,25-dihydroxyvitamin D3 which was contained in the sample or standard.
Kit Component:
ComponentAmount
1,25 DHVD3 Microplate96 Well (12 x 8 Well Strips)
1,25 DHVD3 Standard 5 x 500 uL
High Value Control1 x 500 uL
Middle Value Control1 x 500 uL
Sample Diluent1 x 30 mL
Anti-1,25 DHVD3 Antibody HRP Conjugate1 x 7 mL
20X Wash Buffer1 x 30 mL
Substrate A1 x 7 mL
Substrate B 1 x 7 mL
Stop Solution1 x 7 mL
Kit Linearity:
Sample Type 1
Sample TypeDilutionAverage %Range %
Serum (n=4)1:1085%80-95%
Serum (n=4)1:2092%85-101%
Serum (n=4)1:4093%84-98%
Serum (n=4)1:8096%90-108%
Kit Detection Method:
Colorimetric, OD450 nm
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay has high sensitivity and excellent specificity for detection of rat 1,25
DHVD3. No significant cross-reactivity or interference between rat 1,25 DHVD3
and analogues was observed.
Assay Info:
Assay Methodology: Quantitative Competitive Immunoassay
Additional Information:

CAS#: 32222-06-3

Molecular Formula: C27H44O3

Molecular Weight: 416.63646 g/mol

Protocol:
Tips Information:

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