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| Datasheets/Manuals | Printable datasheet for EMR2 Antibody (OASA01861) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | 2A1 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | Flow cytometry|Immunohistochemistry-Frozen|Immunoprecipitation|Western blot |
| Additional Information | Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. |
| :: | Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant Preservative Stabilisers: 0.09% - Sodium Azide |
| :: | Approx Protein Conc: IgG concentration 1.0 mg/ml Buffer Solutions: Phosphate buffered saline pH7.4 |
| Reconstitution and Storage | -20°C |
| Immunogen | NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA. |
| Purification | Protein A Purified |
| Predicted Homology Based on Immunogen Sequence | Human |
| Concentration | 1 mg/ml |
| Specificity | CD312 |
| Application Info | Flow Cytometry: 1/50 - 1/200 Immunohistology - Frozen: 1/50 - 1/200 |
| Application Data | Application #1: Staining of human peripheral blood granulocytes with Mouse anti Human CD312  Application #2: Staining of human peripheral blood granulocytes with Mouse anti Human CD312: Alexa Fluor 488  Application #3: Staining of human peripheral blood granulocytes with Mouse anti Human CD312: Low Endotoxin  Application #4: Staining of human peripheral blood granulocytes with Mouse anti Human CD312: RPE  Application #5: Staining of human peripheral blood monocytes probed with Mouse anti Human CD312 : Alexa Fluor 647  |
| Reference | 1. Kwakkenbos. M.J. et al. (2002) The human EGF-TM7 family member EMR2 is a heterodimeric receptor expressed on myeloid cells. J. Leukoc. Biol.71:854-862 2. Stacey. M. et al. (2003) The epidermal growth factor-like domains of the human EMR2 receptor mediate cell attachment through chrondroitin sulphate glycosaminoglycans. Blood.102:2916-2924 3. Kwakkenbos. M.J. et al. (2004) The EGF-TM7 family: a postgenomic view. Immunogenetics. 55:655-666 |
| Gene Symbol | EMR2 |
|---|---|
| Alias Symbols | VBU, CD97, EMR2, CD312 |
| NCBI Gene Id | 30817 |
| Protein Name | EGF-like module-containing mucin-like hormone receptor-like 2 |
| Description of Target | MOUSE ANTI HUMAN CD312 |
| Uniprot ID | Q9UHX3 |
| Protein Accession # | NP_038475.2 |
| Protein Size (# AA) | 823 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EMR2 Antibody (OASA01861)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "EMR2 Antibody (OASA01861)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "EMR2 Antibody (OASA01861)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EMR2 Antibody (OASA01861)"?
This target may also be called "VBU, CD97, EMR2, CD312" in publications.
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What is the shipping cost for "EMR2 Antibody (OASA01861)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EMR2 Antibody (OASA01861)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EMR2 Antibody (OASA01861)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EMR2 Antibody (OASA01861)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EMR2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EMR2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EMR2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EMR2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EMR2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EMR2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
