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Catalog No: OCOA01735
Size:20UG
Price: $179.00
SKU
OCOA01735
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EMG1 Protein Lysate (OCOA01735)

Datasheets/ManualsPrintable datasheet for EMG1 Protein Lysate (OCOA01735)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionEMG1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolEMG1
Gene Full NameEMG1 N1-specific pseudouridine methyltransferase
Alias Symbols18S rRNA (pseudouridine(1248)-N1)-methyltransferase;18S rRNA (pseudouridine-N1-)-methyltransferase NEP1;18S rRNA Psi1248 methyltransferase;C2F;EMG1 nucleolar protein homolog;essential for mitotic growth 1;Grcc2f;NEP1;nucleolar essential protein 1;Nucleolar protein EMG1 homolog;Protein C2f;ribosomal RNA small subunit methyltransferase NEP1;ribosome biogenesis protein NEP1.
NCBI Gene Id10436
Protein NameNM_006331, NP_006322, Q92979
Description of TargetHomo sapiens EMG1, N1-specific pseudouridine methyltransferase (EMG1), transcript variant 1, mRNA, Ribosomal RNA small subunit methyltransferase NEP1, ribosomal RNA small subunit methyltransferase NEP1 isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q92979
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_006322
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_006331
Molecular Weight26.7 kDa
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