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Catalog No: OKWB00280
Size:96T
Price: $450.00
SKU
OKWB00280
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Datasheets/ManualsPrintable datasheet for ELOVL7 ELISA Kit (Mouse) (OKWB00280)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-105%86-100%86-103%86-105%
EDTA Plasma (n=5)86-97%83-97%84-101%82-100%
Heparin Plasma (n=5)82-96%80-98%86-99%83-98%
ELISA Kit PrincipleAviva Systems Biology Elovl7 ELISA Kit (Mouse) (OKWB00280) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Elovl7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Elovl7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Elovl7 captured in the well.
ELISA Kit Range78.125-5000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)93-10197
EDTA Plasma (n=5)85-10595
Heparin Plasma (n=5)85-9692
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Elovl7 Microplate96 Wells (12 x 8 Well strips)
Elovl7 Lyophilized Standard2 x 5 ng
100X Elovl7 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity46.9 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolElovl7
Gene Full NameELOVL family member 7, elongation of long chain fatty acids (yeast)
Alias Symbols3-keto acyl-CoA synthase Elovl7;9130013K24Rik;AI840082;elongation of very long chain fatty acids protein 7;elongation of very long chain fatty acids-like 7;ELOVL FA elongase 7;ELOVL fatty acid elongase 7;very long chain 3-ketoacyl-CoA synthase 7;very long chain 3-oxoacyl-CoA synthase 7.
NCBI Gene Id74559
Protein NameElongation of very long chain fatty acids protein 7
Description of TargetCatalyzes the first and rate-limiting reaction of the four that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. Condensing enzyme with higher activity toward C18 acyl-CoAs, especially C18:3(n-3) acyl-CoAs and C18:3(n-6)-CoAs. Also active toward C20:4-, C18:0-, C18:1-, C18:2- and C16:0-CoAs, and weakly toward C20:0-CoA. Little or no activity toward C22:0-, C24:0-, or C26:0-CoAs. May participate in the production of saturated and polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.
Uniprot IDQ9D2Y9
Protein Accession #NP_083277
Nucleotide Accession #NM_029001
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