Catalog No: OKWB00280
Size:96T
Price: $450.00
SKU
OKWB00280
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for ELOVL7 ELISA Kit (Mouse) (OKWB00280) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Elovl7 ELISA Kit (Mouse) (OKWB00280) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Elovl7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Elovl7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Elovl7 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 78.125-5000pg/ml | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Mean Intra-Assay Precision <10% Mean Inter-Assay Precision <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids | ||||||||||||||||||||||
Sensitivity | 46.9 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Type: Quantitative Sandwich ELISA |
Gene Symbol | Elovl7 |
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Gene Full Name | ELOVL family member 7, elongation of long chain fatty acids (yeast) |
Alias Symbols | 3-keto acyl-CoA synthase Elovl7;9130013K24Rik;AI840082;elongation of very long chain fatty acids protein 7;elongation of very long chain fatty acids-like 7;ELOVL FA elongase 7;ELOVL fatty acid elongase 7;very long chain 3-ketoacyl-CoA synthase 7;very long chain 3-oxoacyl-CoA synthase 7. |
NCBI Gene Id | 74559 |
Protein Name | Elongation of very long chain fatty acids protein 7 |
Description of Target | Catalyzes the first and rate-limiting reaction of the four that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. Condensing enzyme with higher activity toward C18 acyl-CoAs, especially C18:3(n-3) acyl-CoAs and C18:3(n-6)-CoAs. Also active toward C20:4-, C18:0-, C18:1-, C18:2- and C16:0-CoAs, and weakly toward C20:0-CoA. Little or no activity toward C22:0-, C24:0-, or C26:0-CoAs. May participate in the production of saturated and polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. |
Uniprot ID | Q9D2Y9 |
Protein Accession # | NP_083277 |
Nucleotide Accession # | NM_029001 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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