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Catalog No: OKEI00446
Size:96 Wells
Price: $625.00
SKU
OKEI00446
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Elmo2 ELISA Kit (Mouse) (OKEI00446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Elmo2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Elmo2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Elmo2
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Elmo2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Elmo2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
Elmo2 Microplate96 Wells (12 x 8 Well strips)
Elmo2 Lyophilized Standard2
100X Elmo2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 10 mL
30X Wash Buffer1 x 30 mL
100X Luminol Substrate1 x 10 mL
Substrate Diluent1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Biological Fluids
Sensitivity0.094 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolELMO2
Alias SymbolsCED-12, 1190002F24Rik
NCBI Gene Id140579
Protein NameEngulfment and cell motility protein 2
Description of TargetInvolved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.
Uniprot IDQ8BHL5
Protein Accession #NP_001289681
Nucleotide Accession #NM_001302752
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