Catalog No: OCOA12713
Size:20UG
Price: $179.00
SKU
OCOA12713
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ELK1 Protein Lysate (OCOA12713)

Datasheets/ManualsPrintable datasheet for ELK1 Protein Lysate (OCOA12713)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionELK1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolELK1
Gene Full NameETS transcription factor ELK1
Alias SymbolsELK1, ETS transcription factor;ELK1, member of ETS oncogene family;ETS domain-containing protein Elk-1;ETS-like gene 1;tyrosine kinase (ELK1) oncogene.
NCBI Gene Id2002
Protein NameA0A024R1A7, NM_005229, NP_005220, P19419
Description of TargetELK1, member of ETS oncogene family, isoform CRA_a, ETS domain-containing protein Elk-1, ETS domain-containing protein Elk-1 isoform a [Homo sapiens], Homo sapiens ELK1, ETS transcription factor (ELK1), transcript variant 2, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/P19419
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_005220
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_005229
Molecular Weight44.9 kDa
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