ELK1 Antibody (OAAD00439)

Catalog No: OAAD00439

Size:0.1 mg

western blot analysis using anti-Human Elk-1 monoclonal antibody against truncated recombinant Elk-1 (
Lane 1) and K562 cell lysate (
Lane 2).

Product Info

• Predicted Species Reactivity: Human
• Clonality: Monoclonal
• Clone: 7E10D5, 7E10E8
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Formulation: Antibodies are purified by protein A affinity chromatogra- phy.
• Application Info: Western Blot: 1: 500- 1: 1, 000 ELISA: Propose dilution 1: 10, 000.
  Determining optimal working dilutions by titration test.
• Reference: 1. Rao, V.N., et al. 1989.Science.244 (4900):66-70 2. Hsieh, Y.H., et al. 2006.Biochem. Biophys. Res. Com- mun. 339 (1): 217-225 3. Gille, H., Strahl, T. and Shaw, P.E.1995. Curr. Biol. 5 (10): 1191-1200 4. Gille, H., et al. 1995.EMBO J. 14 (5): 951-962

Target Info

• Gene Symbol: KCNH4
• Alias Symbols: BEC2, ELK1, Kv12.3
• NCBI Gene Id: 23415
• Protein Name: Potassium voltage-gated channel subfamily H member 4
• Description of Target: The transcription factor ELK1 is a family of member of ETS oncogene family and of the ternary complex factor (TCF) subfamily, which is located on chromosome Xp11.2 and stimulates transcription. binds to purine-rich DNA sequences. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Elk1 is phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C terminus, It appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk1 is a good substrate for MAP kinase, the kinetics of Elk1phosphorylation and activa- tion correlate with MAP kinase activity, and interfering mutants of MAP kinase block Elk1 activation in vivo. More recent studies have shown that Elk1 is also a target of the Stress Activated Kinase SAPK/JNK. Phosphorylation of Elk1 has also been implicated in synaptic plasticity in the adult hippocampus.
• Uniprot ID: Q9UQ05