Catalog No: OPPA01168 (Formerly GWB-598916)
Size:2UG
Price: $75.00
SKU
OPPA01168
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA01168 |
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Predicted Species Reactivity | Human |
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Product Format | ELK-1 is supplied as lyophilized freeze dry powder without additives. |
Host | E. Coli |
Additional Information | Product Note: Kinase activity may vary depending on the substrate and reaction conditions. |
:: | Biological Activity: Recombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application. |
:: | Product Introduction: Elk-1 is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Because of the importance of Elk-1 to the regulation of successful cell growth, accurate monitoring of Elk-1 in cells, tissues and animals is crucial for many biomedical research and drug development projects. Product Description: ELK-1 (amino acids 307-428) Human Recombinant is produced in E.Coli as a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and a c-terminal myc tag (EQKLISEEDL), and having a molecular mass of 60 kDa. Elk-1 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Store at 2-8C if entire vial will be used within 1-2 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles. |
Purity | Greater than 95% as determined by SDS-PAGE. |
Gene Symbol | ELK-1 |
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Alias Symbols | ETS domain-containing protein Elk-1, ELK1, Elk-1, Elk-1 Human, Elk-1 Human Recombinant |
NCBI Gene Id | 2002 |
Protein Name | ETS domain-containing protein Elk-1 |
Description of Target | Recombinant Human ELK-1 |
Uniprot ID | P19419 |
Protein Accession # | NP_001107595.1 |
Protein Size (# AA) | Active|Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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