ELISA Ultrablock Accessory Reagent (OOSA09552)

Catalog No: OOSA09552 (Formerly GWB-Q00227)

Size:100ML

Product Info

• Product Format: Liquid. Phosphate buffered saline
• Application: Enzyme-linked immunosorbent assay
• Additional Information: Buffer solution: Phosphate buffered saline
  Preservative: 0.05% - Sodium Azide
  Intended Use: 
  OOSA09552 is a high performance ELISA blocking buffer for use in all ELISA formats with high background problems. The formulation includes a proprietary fish extract, molecular stabilizers and an antimicrobial agent. It is particularly useful in antigen-down and sandwich ELISA assays with mammalian samples, especially human, bovine and porcine serum, where the non-mammalian blocking proteins are less likely to interact with endogenous antibodies in the serum sample.
  
  

  Superior blocking is possible due to the small size of the molecules which block non-specific binding sites on the adsorbed protein and unoccupied regions of the polystyrene plates often sterically inaccessible to traditional blockers. It provides a long-term stable environment for coating antigen or capture antibody. Plates can be blocked at room temperature and stored once dried for up to a year at +4C.

  
  Instructions for Use:
  1. Coat ELISA plate with antibody or antigen as required. 
  2. After incubation, remove the coating solution and wash the plate x2 with wash buffer.  OOSA09546 can be used for this purpose.
  3. Add 300-400 ul of OOSA09552 and incubate for 2-24 hours.  Use a volume equal to or greater than the volume of coating solution.
  4. After removal of the blocking buffer, continue with the assay of dry the plate for long-term storage at +4C. 
• Reconstitution and Storage: 2°C to 8°C
• Application Info: ELISA: Neat
  Direct and Indirect/sandwich

Target Info

• Description of Target: ELISA Ultrablock