ELISA IgM REDUCING BUFFER Accessory Reagent (OOSA09561)

Catalog No: OOSA09561 (Formerly GWB-Q00218)

Size:100ML

Product Info

• Product Format: Liquid. Phosphate buffered saline
• Application: Enzyme-linked immunosorbent assay
• Reconstitution and Storage: 2°C to 8°C
• Application Info: ELISA: Neat
• Procedure: Pipette buffer directly onto the plate, just prior to adding the sample or standard (suggested volume 50 - 100 ul/well).
  Since this buffer contains proteins derived from goat, OOSA09561 is not recommended for ELISA where an anti goat secondary antibody is used.
• Description: OOSA09561 is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of "sticky" IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface.
  The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. OOSA09561 equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.

Target Info

• Description of Target: OOSA09561 is an ELISA assay diluent designed to reduce background noise and assist in matrix equalization in problematic serum and plasma samples, especially those affected by high levels of “sticky” IgM which can non-specifically bind the HRP conjugate. Specially formulated for use in sandwich ELISA, it contains mammalian protein additives based on normal goat serum to reduce non-specific interactions between sample proteins and the plate surface. The difference in complexity, between serum or plasma samples and diluted standard curve proteins, is a potential cause of target analyte under-recovery. OOSA09561 equalizes the matrix, reduces IgM-mediated bridging interference, inhibits complement and thrombin activity and contains an antimicrobial agent.