ELAVL1 ELISA Kit (Rat) (OKCD09220)

Catalog No: OKCD09220

Size:96WELLS

ELAVL1 ELISA Kit (Rat) (OKCD09220) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELAV Like Protein 1 (ELAVL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ELAV Like Protein 1 (ELAVL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELAV Like Protein 1 (ELAVL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ELAV Like Protein 1 (ELAVL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELAV Like Protein 1 (ELAVL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ELAVL1 Microplate: 96 Wells (12 x 8 Well strips)
  • ELAVL1 Lyophilized Standard: 2
  • 100X ELAVL1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.066ng/mL

Target Info

• Gene Symbol: Elavl1
• Gene Full Name: ELAV like RNA binding protein 1
• Alias Symbols: ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R);ELAV like 1;ELAV-like protein 1;Hua;hu-antigen R;HuR;mRNA-stabilizing factor HuR.
• NCBI Gene Id: 363854
• Protein Name: ELAV-like protein 1
• Description of Target: RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (By similarity). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (By similarity). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity).
• Uniprot ID: B5DF91
• Protein Accession #: NP_001102318.1
• Nucleotide Accession #: NM_001108848.1