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Catalog No: OKDD00762 (Formerly GWB-KBBLL3)
Size:96 Wells
Price: $850.00
SKU
OKDD00762
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Datasheets/ManualsPrintable datasheet for ELA3B ELISA Kit (Rat) (OKDD00762)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology ELA3B ELISA Kit (Rat) (OKDD00762) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for ELA3B has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ELA3B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ELA3B captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-ELA3B Microplate96 Wells (12 x 8 Well Strips)
ELA3B Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X ELA3B Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates
Sensitivity<0.053ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of ELA3B. No significant cross-reactivity or interference between ELA3B and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolCela3b
Gene Full Namechymotrypsin like elastase 3B
Alias Symbolschymotrypsin-like elastase family member 3B;chymotrypsin-like elastase family, member 3B;Ela3b;elastase 3B, pancreatic.
NCBI Gene Id298567
Protein NameChymotrypsin-like elastase family, member 3B
Uniprot IDD3ZFG3
Protein Accession #NP_001100162.1
Nucleotide Accession #NM_001106692.1
Protein Size (# AA)269
Molecular Weight29 kDa
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