Catalog No: OKCD04434
Size:96WELLS
Price: $700.00
SKU
OKCD04434
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Datasheets/ManualsPrintable datasheet for ELA2 ELISA Kit (Dog) (OKCD04434)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)98-105%79-97%80-102%79-90%
EDTA Plasma (n=5)98-105%89-102%96-105%78-94%
Heparin Plasma (n=5)80-90%95-103%80-89%92-99%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Elastase (NE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neutrophil Elastase (NE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Elastase (NE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Neutrophil Elastase (NE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)88-9591
EDTA Plasma (n=5)88-9892
Heparin Plasma (n=5)78-10582
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neutrophil Elastase (NE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neutrophil Elastase (NE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ela2 Microplate96 Wells (12 x 8 Well strips)
Ela2 Lyophilized Standard2
100X Ela2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity13.5 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolELANE
Gene Full Nameelastase, neutrophil expressed
Alias SymbolsELA2;elastase 2, neutrophil;neutrophil elastase;serine protease.
NCBI Gene Id442980
Protein NameElastase, neutrophil expressed
Description of TargetElastase, neutrophil expressed
Uniprot IDQ8MJD1
Protein Accession #NP_001003378.1
Nucleotide Accession #NM_001003378.2
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