EIF5A ELISA Kit (Human) (OKEH08524)

Catalog No: OKEH08524

Size:96T

Human EIF5A ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology EIF5A ELISA Kit (Human) (OKEH08524) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EIF5A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EIF5A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EIF5A captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.8% (n = 20)
  Mean Inter-assay CV%: <=9.1% (n = 20)
• ELISA Kit Component:
  • EIF5A Microplate: 96 Wells (12 x 8 Well strips)
  • EIF5A Lyophilized Standard: 2
  • 100X Biotinylated EIF5A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
  Subcellular Location: Cytoplasm Nucleus Endoplasmic reticulum membrane Peripheral membrane protein Cytoplasmic side Nucleus Nuclear pore complex Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
  Unigene: Hs.104825
  SMR: P63241
  String: 9606.ENSP00000336702
  KEGG: hsa:1984
  MIM: 600187
  Pfam: PF01287
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.163ng/mL
• Specificity: Natural and recombinant Human Eukaryotic translation initiation factor 5A-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EIF5A
• Gene Full Name: eukaryotic translation initiation factor 5A
• Alias Symbols: eIF-4D;EIF-5A;EIF5A1;eIF5AI;eukaryotic initiation factor 5A;Eukaryotic initiation factor 5A isoform 1;eukaryotic translation initiation factor 5A-1;FABAS;rev-binding factor.
• NCBI Gene Id: 1984
• Protein Name: P63241
• Description of Target: Eukaryotic translation initiation factor 5A-1
• Uniprot ID: https://www.uniprot.org/uniprot/P63241
• Protein Accession #: NP_001137232.1
• Nucleotide Accession #: NM_001143760.1