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Datasheets/Manuals | Printable datasheet for anti-EIF4E (ARP40377_P050) antibody |
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Tested Species Reactivity | Human | ||||||
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish | ||||||
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. | ||||||
Clonality | Polyclonal | ||||||
Host | Rabbit | ||||||
Application | IHC, WB | ||||||
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. | ||||||
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human EIF4E | ||||||
Purification | Affinity Purified | ||||||
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100% | ||||||
Peptide Sequence | Synthetic peptide located within the following region: TECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV | ||||||
Concentration | 0.5 mg/ml | ||||||
Blocking Peptide | For anti-EIF4E (ARP40377_P050) antibody is Catalog # AAP40377 (Previous Catalog # AAPP22121) | ||||||
Enhanced Validation |
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Reference | Lee,H.C., (2008) Biochem. Biophys. Res. Commun. 369 (4), 1160-1165 |
Gene Symbol | EIF4E |
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Gene Full Name | Eukaryotic translation initiation factor 4E |
Alias Symbols | CBP, EIF4F, AUTS19, EIF4E1, eIF-4E, EIF4EL1 |
NCBI Gene Id | 1977 |
Protein Name | Eukaryotic translation initiation factor 4E |
Description of Target | All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Uniprot ID | P06730 |
Protein Accession # | NP_001959 |
Nucleotide Accession # | NM_001968 |
Protein Size (# AA) | 217 |
Molecular Weight | 25 kDa |
Protein Interactions | EIF4EBP1; UBXN11; EIF4ENIF1; KANK2; TRIM22; EIF4EBP3; TDO2; TRIM27; EIF4G1; EIF4EBP2; EIF3B; RPS6KB1; MTOR; PML; NANS; PROSC; AHSA1; PEPD; EIF6; HSPD1; HSPA9; TYMP; CAPN1; ASS1; GEMIN5; PRDM4; CD83; UBC; ITGA4; APP; EIF4H; RPTOR; EIF4A1; RPS6KA5; HSPB2; E |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EIF4E Antibody - C-terminal region (ARP40377_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish".
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How long will it take to receive "EIF4E Antibody - C-terminal region (ARP40377_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "EIF4E Antibody - C-terminal region (ARP40377_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EIF4E Antibody - C-terminal region (ARP40377_P050)"?
This target may also be called "CBP, EIF4F, AUTS19, EIF4E1, eIF-4E, EIF4EL1" in publications.
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What is the shipping cost for "EIF4E Antibody - C-terminal region (ARP40377_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EIF4E Antibody - C-terminal region (ARP40377_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EIF4E Antibody - C-terminal region (ARP40377_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "25 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EIF4E Antibody - C-terminal region (ARP40377_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EIF4E"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EIF4E"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EIF4E"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EIF4E"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EIF4E"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EIF4E"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.