EIF4A1 Protein Lysate (OCOA00485)

Catalog No: OCOA00485

Size:20UG

Product Info

• Predicted Species Reactivity: Homo sapiens
• Product Format: Lyophilized
• Host: HEK293T
• Additional Information: Components: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
  Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
• Reconstitution and Storage: The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
• Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
• Tag: C-MYC/DDK
• ELISA Kit Component: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
• Description: EIF4A1 HEK293T cell transient overexpression lysate (as WB positive control)

Target Info

• Gene Symbol: EIF4A1
• Gene Full Name: eukaryotic translation initiation factor 4A1
• Alias Symbols: ATP-dependent RNA helicase eIF4A-1;DDX2A;EIF4A;EIF-4A;eIF4A-I;eIF-4A-I;eukaryotic initiation factor 4AI;eukaryotic initiation factor 4A-I;eukaryotic translation initiation factor 4A.
• NCBI Gene Id: 1973
• Protein Name: NM_001416, NP_001407, P60842
• Description of Target: Eukaryotic initiation factor 4A-I, eukaryotic initiation factor 4A-I isoform 1 [Homo sapiens], Homo sapiens eukaryotic translation initiation factor 4A1 (EIF4A1), transcript variant 1, mRNA
• Uniprot ID: https://www.uniprot.org/uniprot/P60842
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001407
• Nucleotide Accession #: https://www.ncbi.nlm.nih.gov/nuccore/NM_001416
• Molecular Weight: 46.2 kDa