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Catalog No: OCOA12154
Size:20UG
Price: $179.00
SKU
OCOA12154
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EIF3M Protein Lysate (OCOA12154)

Datasheets/ManualsPrintable datasheet for EIF3M Protein Lysate (OCOA12154)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionEIF3M HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolEIF3M
Gene Full Nameeukaryotic translation initiation factor 3 subunit M
Alias SymbolsB5;B5 receptor;dendritic cell protein;eukaryotic translation initiation factor 3 subunit M;fetal lung protein B5;GA17;hfl-B5;PCI domain containing 1 (herpesvirus entry mediator);PCI domain-containing protein 1;PCID1;TANGO7;transport and golgi organization 7 homolog.
NCBI Gene Id10480
Protein NameNM_006360, NP_006351, Q7L2H7
Description of TargetEukaryotic translation initiation factor 3 subunit M, eukaryotic translation initiation factor 3 subunit M isoform 1 [Homo sapiens], Homo sapiens eukaryotic translation initiation factor 3 subunit M (EIF3M), transcript variant 1, mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q7L2H7
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_006351
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_006360
Molecular Weight42.5 kDa
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