EIF3F Antibody (OAED00076)

Catalog No: OAED00076

Size:50 ug

Western blot analysis of mouse 3T3 cell lysate, probed with KAP-CP130

IHC Analysis: Human pancreas tissue stained with eIF2alpha, pAb at 10 ug/ml.

IHC Analysis: Human prostate tissue stained with eIF2 alpha, pAb at 10 ug/ml.

IHC Analysis: Human vessel tissue stained with eIF2 alpha, pAb at 10 ug/ml.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Guinea Pig|Hamster|Human|Monkey|Mouse|Porcine|Rabbit|Rat|Sheep
• Clonality: Polyclonal
• Host: Rabbit
• Application: Immunocytochemistry|Western blot
• Reconstitution and Storage: Long Term Storage : -20C
  
• Immunogen: Synthetic peptide corresponding to a portion of rat eIF2-alpha.
• Specificity: Recognizes human, mouse, rat, bovine, dog, guinea pig, hamster, monkey, pig, rabbit and sheep eIF2-alpha. Detects a band of ~36kDa by Western blot.
• Formulation: Liquid. In PBS, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
• Purity: Immunoaffinity purified.
  
• Application Info: Immunocytochemistry
  Immunohistochemistry (paraffin sections)
  Western Blot (1μg/ml, ECL)
  Optimal conditions must be determined individually for each application.

Target Info

• Reference: Prolonged maternal amino acid infusion in late-gestation pregnant sheep increases fetal amino acid oxidation: L. Brown, et al. ; Am. J. Physiol. Endocrinol. Metab. 297, E638 (2009), Application(s): WB using sheep tissue, Abstract;
  Evidence that ternary complex (eIF2-GTP-tRNA(i)(Met))-deficient preinitiation complexes are core constituents of mammalian stress granules: P. Anderson, et al. ; Mol. Biol. Cell 13, 195 (2002), Application(s): ICC, WB using human samples, Abstract;
  PERK mediates cell-cycle exit during the mammalian unfolded protein response: J.A. Diehl, et al. ; PNAS 97, 12625 (2000), Application(s): WB using mouse samples, Abstract;
  
• Gene Symbol: Eif2s1
• Gene Full Name: eukaryotic translation initiation factor 2 subunit alpha
• Alias Symbols: EIF-2A;eIF-2-alpha;EIF-2alpha;eukaryotic translation initiation factor 2 subunit 1;eukaryotic translation initiation factor 2 subunit 1 alpha;Eukaryotic translation initiation factor 2 subunit alpha.
• NCBI Gene Id: 54318
• Protein Name: Eukaryotic translation initiation factor 2 subunit 1
• Description of Target: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
• Uniprot ID: P68101
• Protein Accession #: NP_003745.1
• Nucleotide Accession #: NM_003754.2