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Datasheets/Manuals | Printable datasheet for EIF2S1 Antibody (OAAF01286) |
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Predicted Species Reactivity | Human|Monkey|Mouse|Rat |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human eIF2 alpha. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | eIF2α (Ab-51) antibody detects endogenous levels of total eIF2α protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:10000 |
Reference | Xavier Saelens, et,al. (2001) J. Biol. Chem; 276: 41620-41628. Hiroyuki Kubota,et,al.(2003) J. Biol. Chem ; 278: 20457-20460. Shijian Chu, et,al. (2006) Am J Physiol Lung Cell Mol Physiol ; 291: L983-L992. Eileen Connolly, et,al.(2006) Mol. Cell. Biol ; 26: 3955-3965. |
Gene Symbol | EIF2S1 |
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Gene Full Name | eukaryotic translation initiation factor 2 subunit alpha |
Alias Symbols | EIF2;EIF-2;EIF2A;EIF-2A;eIF-2-alpha;EIF-2alpha;eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa. |
NCBI Gene Id | 1965 |
Protein Name | Eukaryotic translation initiation factor 2 subunit 1 |
Description of Target | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
Uniprot ID | P05198 |
Protein Accession # | NP_004085.1 |
Molecular Weight | 36 kda |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EIF2S1 Antibody (OAAF01286)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Monkey|Mouse|Rat".
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How long will it take to receive "EIF2S1 Antibody (OAAF01286)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "EIF2S1 Antibody (OAAF01286)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EIF2S1 Antibody (OAAF01286)"?
This target may also be called "EIF2;EIF-2;EIF2A;EIF-2A;eIF-2-alpha;EIF-2alpha;eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa." in publications.
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What is the shipping cost for "EIF2S1 Antibody (OAAF01286)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EIF2S1 Antibody (OAAF01286)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EIF2S1 Antibody (OAAF01286)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "36 kda".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EIF2S1 Antibody (OAAF01286)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EIF2S1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EIF2S1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EIF2S1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EIF2S1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.