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Datasheets/Manuals | Printable datasheet for anti-EIF2S1 (OAAB21173) antibody |
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Predicted Species Reactivity | Human, Mouse |
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Product Format | Liquid |
Clonality | Monoclonal |
Host | Mouse |
Application | WB |
Additional Information | Function: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Predicted Homology Based on Immunogen Sequence | Human, Mouse |
Preservative | 0.03% Proclin300 and 50% glycerol |
Formulation | PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol. |
Reference | Ernst H.,et al.J. Biol. Chem. 262:1206-1212(1987). Langland J.O.,et al.Virology 324:419-429(2004). Paytubi S.,et al.Biochem. J. 409:223-231(2008). Montero H.,et al.J. Virol. 82:1496-1504(2008). Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009). |
Gene Symbol | EIF2S1 |
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Gene Full Name | eukaryotic translation initiation factor 2 subunit alpha |
Alias Symbols | EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha |
NCBI Gene Id | 1965 |
Description of Target | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
Uniprot ID | P05198 |
Protein Accession # | NP_004085.1 |
Nucleotide Accession # | NM_004094.4 |
Molecular Weight | 36 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EIF2S1 Antibody (OAAB21173)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
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How long will it take to receive "EIF2S1 Antibody (OAAB21173)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "EIF2S1 Antibody (OAAB21173)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EIF2S1 Antibody (OAAB21173)"?
This target may also be called "EIF2, EIF-2, EIF2A, EIF-2A, EIF-2alpha" in publications.
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What is the shipping cost for "EIF2S1 Antibody (OAAB21173)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EIF2S1 Antibody (OAAB21173)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EIF2S1 Antibody (OAAB21173)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "36 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EIF2S1 Antibody (OAAB21173)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EIF2S1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EIF2S1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EIF2S1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EIF2S1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.