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Datasheets/Manuals | Printable datasheet for EIF2S1 Antibody - middle region (OABB01181) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Hamster|Human |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: WB: The detection limit for EIF2S1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: EIF2S1(Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence(GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Polypeptide |
Purification | Affinity Purified |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human. |
Gene Symbol | EIF2S1 |
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Gene Full Name | eukaryotic translation initiation factor 2 subunit alpha |
Alias Symbols | EIF2;EIF-2;EIF2A;EIF-2A;eIF-2-alpha;EIF-2alpha;eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa. |
NCBI Gene Id | 1965 |
Protein Name | Eukaryotic translation initiation factor 2 subunit 1 |
Description of Target | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
Uniprot ID | P05198 |
Molecular Weight | 36112 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "EIF2S1 Antibody - middle region (OABB01181)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Hamster|Human".
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How long will it take to receive "EIF2S1 Antibody - middle region (OABB01181)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "EIF2S1 Antibody - middle region (OABB01181)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "EIF2S1 Antibody - middle region (OABB01181)"?
This target may also be called "EIF2;EIF-2;EIF2A;EIF-2A;eIF-2-alpha;EIF-2alpha;eukaryotic translation initiation factor 2 subunit 1;Eukaryotic translation initiation factor 2 subunit alpha;eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa." in publications.
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What is the shipping cost for "EIF2S1 Antibody - middle region (OABB01181)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "EIF2S1 Antibody - middle region (OABB01181)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "EIF2S1 Antibody - middle region (OABB01181)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "36112 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "EIF2S1 Antibody - middle region (OABB01181)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "EIF2S1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "EIF2S1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "EIF2S1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "EIF2S1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "EIF2S1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.