Catalog No: OCOA13378
Size:20UG
Price: $179.00
SKU
OCOA13378
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EIF2B5 Protein Lysate (OCOA13378)

Datasheets/ManualsPrintable datasheet for EIF2B5 Protein Lysate (OCOA13378)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionEIF2B5 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolEIF2B5
Gene Full Nameeukaryotic translation initiation factor 2B subunit epsilon
Alias SymbolsCACH;CLE;EIF-2B;eIF-2B GDP-GTP exchange factor subunit epsilon;EIF2Bepsilon;eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa;LVWM;translation initiation factor eIF-2B subunit epsilon.
NCBI Gene Id8893
Protein NameNM_003907, NP_003898, Q13144
Description of TargetHomo sapiens eukaryotic translation initiation factor 2B subunit epsilon (EIF2B5), mRNA, Translation initiation factor eIF-2B subunit epsilon, translation initiation factor eIF-2B subunit epsilon [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q13144
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_003898
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_003907
Molecular Weight80.4 kDa
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