EIF2AK4 Antibody (Phospho-Thr667) (OABF01173)

Catalog No: OABF01173

Size:100UL

Formalin-fixed and paraffin embedded mouse liver labeled with Rabbit Anti phospho-GCN2 (Thr667) Polyclonal Antibody, Unconjugated (OABF01173) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Product Info

• Predicted Species Reactivity: Bovine|Dog|Horse|Human|Mouse|Rat
• Product Format: Liquid Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
  Modification: Phosphorylation (Thr667)
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic phosphopeptide derived from human GCN2 around the phosphorylation site of Thr667
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Specificity: This phosphorylation site is homologous to Thr666 in Mouse and Thr667 in Rat
• Application Info: WB: 1:300-1000
  IHC-P: 1:200-400
  Fluorescent-IHC: 1:50-200
  Optimal dilutions should be determined by the end user.
• Publications:

  Effects of the presence and absence of amino acids on translation, signaling, and long-term depression in hippocampal slices from Fmr1 knockout mice. J Neurochem. 151, 764-776 (2019). 31539452

• Description:
  View product citations for EIF2AK4 Antibody (Phospho-Thr667) (OABF01173) on CiteAb

Target Info

• Gene Symbol: EIF2AK4
• Gene Full Name: eukaryotic translation initiation factor 2 alpha kinase 4
• Alias Symbols: eIF-2-alpha kinase GCN2;Eukaryotic translation initiation factor 2-alpha kinase 4;GCN2;GCN2 eIF2alpha kinase;GCN2-like protein;general control nonderepressible 2;PVOD2.
• NCBI Gene Id: 440275
• Protein Name: eIF-2-alpha kinase GCN2
• Description of Target: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' in response to low amino acid availability (PubMed:25329545). Plays a role as an activator of the integrated stress response (ISR) required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha/EIF2S1 either to a competitive inhibitor of the translation initiation factor eIF-2B, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs (By similarity). Involved in cell cycle arrest by promoting cyclin D1 mRNA translation repression after the unfolded protein response pathway (UPR) activation or cell cycle inhibitor CDKN1A/p21 mRNA translation activation in response to amino acid deprivation (PubMed:26102367). Plays a role in the consolidation of synaptic plasticity, learning as well as formation of long-term memory. Plays a role in neurite outgrowth inhibition. Plays a proapoptotic role in response to glucose deprivation. Promotes global cellular protein synthesis repression in response to UV irradiation independently of the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) and p38 MAPK signaling pathways (By similarity). Plays a role in the antiviral response against alphavirus infection; impairs early viral mRNA translation of the incoming genomic virus RNA, thus preventing alphavirus replication (By similarity).
• Uniprot ID: Q9P2K8