EIF2AK4 Antibody - C-terminal region (OAGA03779)

Catalog No: OAGA03779

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
B: Molt-4
5% SDS PAGE
diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using GCN2 antibody at 1:500 dilution.

GCN2 antibody [C2C3], C-term detects EIF2AK4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: EIF2AK4 protein stained by GCN2 antibody [C2C3], C-term diluted at 1:500.
Blue: Hoechst 33343 staining.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human GCN2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.7 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: EIF2AK4
• Gene Full Name: eukaryotic translation initiation factor 2 alpha kinase 4
• Alias Symbols: GCN2, PVOD2
• NCBI Gene Id: 440275
• Protein Name: eIF-2-alpha kinase GCN2
• Description of Target: This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
• Uniprot ID: Q9P2K8
• Protein Accession #: NP_001013725.2
• Nucleotide Accession #: NM_001013703.3