Catalog No: OKEH04633
Size:96T
Price: $525.00
SKU
OKEH04633
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Eif2ak2 ELISA Kit (Rat) (OKEH04633) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eif2ak2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Eif2ak2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eif2ak2 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Eif2ak2 Microplate96 Wells (12 x 8 Well Strips)
Eif2ak2 Lyophilized Standard2
Sample Diluent1 x 20 mL
Eif2ak2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.158 ng/mL
SpecificityNatural and recombinant Rat Interferon-induced, double-stranded RNA-activated protein kinase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEif2ak2
Gene Full Nameeukaryotic translation initiation factor 2-alpha kinase 2
Alias SymbolseIF-2A protein kinase 2;Eukaryotic translation initiation factor 2-alpha kinase 2;interferon-induced, double-stranded RNA-activated protein kinase;interferon-inducible RNA-dependent protein kinase;Pkr;Prkr;protein kinase R;protein kinase RNA-activated;Protein kinase, interferon-inducible double stranded RNA dependent;tyrosine-protein kinase EIF2AK2.
NCBI Gene Id54287
Protein NameQ63184
Description of TargetInterferon-induced, double-stranded RNA-activated protein kinase
Uniprot IDhttps://www.uniprot.org/uniprot/Q63184
Protein Accession #NP_062208.1
Nucleotide Accession #NM_019335.1
Protein Size (# AA)516
Molecular Weight58 KDa
Write Your Own Review
You're reviewing:Eif2ak2 ELISA Kit (Rat) (OKEH04633)
Your Rating
We found other products you might like!