Catalog No: OKEH05663
Size:96T
Price: $525.00
SKU
OKEH05663
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Predicted Species Reactivity | Mouse, Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Eif2ak2 ELISA Kit (Mouse) (OKEH05663) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Eif2ak2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Eif2ak2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Eif2ak2 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 91.7% | ||||||||||||||||||||||
ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <8.3%, n = 20 Mean Inter-Assay Precision - <10.0%, n = 20 | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.157 ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Mouse Interferon-induced, double-stranded RNA-activated protein kinase | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | Eif2ak2 |
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Gene Full Name | eukaryotic translation initiation factor 2-alpha kinase 2 |
Alias Symbols | 2310047A08Rik;4732414G15Rik;AI467567;AI747578;dsRNA-activated kinase;eIF-2 alpha;eIF-2A protein kinase 2;Eukaryotic translation initiation factor 2-alpha kinase 2;IFN- type I-induced and dsRNA-activated kinase;IFN-induced and double-stranded RNA-activated kinase;interferon-induced, double-stranded RNA-activated protein kinase;interferon-inducible RNA-dependent protein kinase;P;P1/eIF-2A protein kinase;p68 kinase;Pkr;Prkr;protein kinase R;protein kinase RNA-activated;protein kinase, interferon-inducible double stranded RNA dependent;serine/threonine-protein kinase TIK;T-cell viral integration site;Ti;Tik;tyrosine-protein kinase EIF2AK2. |
NCBI Gene Id | 19106 |
Protein Name | Q03963 |
Description of Target | Interferon-induced, double-stranded RNA-activated protein kinase |
Uniprot ID | https://www.uniprot.org/uniprot/Q03963 |
Protein Accession # | NP_035293.1 |
Nucleotide Accession # | NM_011163.4 |
Protein Size (# AA) | 515 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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