Catalog No: OKEH00802
Size:96W
Price: $555.00
SKU
OKEH00802
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Datasheets/ManualsPrintable datasheet for EIF2AK2 ELISA Kit (Human) (OKEH00802)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology EIF2AK2 ELISA Kit (Human) (OKEH00802) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EIF2AK2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EIF2AK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EIF2AK2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.0% (n = 20)
Mean Inter-assay CV%: < 9.8% (n = 20)
ELISA Kit Component
ComponentAmount
EIF2AK2 Microplate96 Wells (12 x 8 Well strips)
EIF2AK2 Lyophilized Standard2
100X Biotinylated EIF2AK2 Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.045 ng/mL
SpecificityNatural and recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolEIF2AK2
Gene Full Nameeukaryotic translation initiation factor 2 alpha kinase 2
Alias Symbolsdouble stranded RNA activated protein kinase, eIF-2A protein kinase 2, EIF2AK1, Eukaryotic translation initiation factor 2-alpha kinase 2, interferon-induced, double-stranded RNA-activated protein kinase, interferon-inducible elF2alpha kinase, Interferon-inducible RNA-dependent protein kinase, LEUDEN, P1/eIF-2A protein kinase, p68 kinase, PKR, PPP1R83, PRKR, protein kinase R, Protein kinase RNA-activated, protein kinase, interferon-inducible double stranded RNA dependent, protein phosphatase 1, regulatory subunit 83, tyrosine-protein kinase EIF2AK2.
NCBI Gene Id5610
Protein NameInterferon-induced, double-stranded RNA-activated protein kinase
Description of TargetIFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes. Can trigger apoptosis via FADD-mediated activation of CASP8. Plays a role in the regulation of the cytoskeleton by binding to gelsolin (GSN), sequestering the protein in an inactive conformation away from actin.
Uniprot IDP19525
Protein Accession #NP_001129123.1
Nucleotide Accession #NM_001135651.2
Protein Size (# AA)551
Molecular Weight62 kDa
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