EIF2AK2 ELISA Kit (Human) (OKEH00802)

Catalog No: OKEH00802

Size:96T

Human EIF2AK2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology EIF2AK2 ELISA Kit (Human) (OKEH00802) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EIF2AK2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EIF2AK2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample EIF2AK2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.0% (n = 20)
  Mean Inter-assay CV%: < 9.8% (n = 20)
• ELISA Kit Component:
  • EIF2AK2 Microplate: 96 Wells (12 x 8 Well strips)
  • EIF2AK2 Lyophilized Standard: 2
  • 100X Biotinylated EIF2AK2 Detector Antibody: 1 x 120 ÂuL
  • 100X Avidin-HRP Conjugate: 1 x 120 ÂuL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.045 ng/mL
• Specificity: Natural and recombinant Human Interferon-induced, double-stranded RNA-activated protein kinase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EIF2AK2
• Gene Full Name: eukaryotic translation initiation factor 2 alpha kinase 2
• Alias Symbols: double stranded RNA activated protein kinase;eIF-2A protein kinase 2;EIF2AK1;Eukaryotic translation initiation factor 2-alpha kinase 2;interferon-induced, double-stranded RNA-activated protein kinase;interferon-inducible elF2alpha kinase;Interferon-inducible RNA-dependent protein kinase;LEUDEN;P1/eIF-2A protein kinase;p68 kinase;PKR;PPP1R83;PRKR;protein kinase R;Protein kinase RNA-activated;protein kinase, interferon-inducible double stranded RNA dependent;protein phosphatase 1, regulatory subunit 83;tyrosine-protein kinase EIF2AK2.
• NCBI Gene Id: 5610
• Protein Name: P19525
• Description of Target: Interferon-induced, double-stranded RNA-activated protein kinase
• Uniprot ID: https://www.uniprot.org/uniprot/P19525
• Protein Accession #: NP_001129123.1
• Nucleotide Accession #: NM_001135651.2
• Protein Size (# AA): 551
• Molecular Weight: 62 kDa