EIF2AK2 Antibody (Phospho-Thr446) (OAAJ02505)

Catalog No: OAAJ02505

Size:100 ul

Western blot analysis of PKR phosphorylation expression in Starvation treated K562 whole cell lysates. The lane on the left is treated with the antigen-specific peptide.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IF, ICC, IHC
• Additional Information: Subcellular Location: Nucleus
  Tissue Specificity: Highly expressed in thymus, spleen and bone marrow compared to non-hematopoietic tissues such as small intestine, liver, or kidney tissues. Colocalizes with GSK3B and TAU in the Alzheimer disease (AD) brain. Elevated levels seen in breast and colon carcinomas,and which correlates with tumor progression and invasiveness or risk of progression.
  Similarity: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily
• Reconstitution and Storage: Store at -20°C. Stable for 12 months from date of receipt.
• Immunogen: A synthesized peptide derived from human PKR around the phosphorylation site of Thr446
• Purification: The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and nonphospho-peptide affinity columns.
• Concentration: 1 mg/ml
• Specificity: Phospho-PKR (Thr446) Ab detects endogenous levels of PKR only when phosphorylated at Threonine 446
• Application Info: WB: 1:500-1:2000; For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
  IF: 1:100-1:500
  ICC: 1:100-1:500
  IHC: 1:50-1:200
  Optimal dilutions should be determined by the end user.

Target Info

• Gene Symbol: EIF2AK2
• Gene Full Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Alias Symbols: PKR, PRKR, LEUDEN, EIF2AK1, PPP1R83
• NCBI Gene Id: 5610
• Description of Target: PKR a protein kinase of the PEK family. Upon binding doublestranded RNA, it becomes autophosphorylated and activated. Phosphorylates and inhibits the alpha subunit of eIF2 alpha, which leads to an inhibition of the initiation of protein synthesis.
• Uniprot ID: P19525
• Molecular Weight: 74 kDa