EIF2AK2 Antibody - middle region (ARP36709_P050)

Catalog#: ARP36709_P050

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Replacement Item: This antibody may replace item sc-100378 from Santa Cruz Biotechnology.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EIF2AK2
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 83%; Horse: 91%; Human: 100%; Mouse: 83%; Pig: 83%; Rabbit: 100%; Rat: 92%
• Peptide Sequence: Synthetic peptide located within the following region: QVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDG
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Blocking Peptide: For anti-EIF2AK2 (ARP36709_P050) antibody is Catalog # AAP36709 (Previous Catalog # AAPP08602)
• Sample Type Confirmation:

  EIF2AK2 is strongly supported by BioGPS gene expression data to be expressed in HeLa

• Gene Symbol: EIF2AK2
• Official Gene Full Name: Eukaryotic translation initiation factor 2-alpha kinase 2
• Alias Symbols: EIF2AK1, MGC126524, PKR, PRKR
• NCBI Gene Id: 5610
• Protein Name: Interferon-induced, double-stranded RNA-activated protein kinase
• Description of Target: EIF2AK2 might play a role in ER stress-induced apoptosis and in Alzheimer's disease. Alzheimer cases show prominent EIF2AK2 activation in association with neuritic plaques and pyramidal neurons in the hippocampus and neocortex.
• Swissprot Id: P19525
• Protein Accession #: NP_002750
• Nucleotide Accession #: NM_002759
• Protein Size (# AA): 551
• Molecular Weight: 62kDa
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express EIF2AK2.
• Protein Interactions: UBC; TRAF6; TRAF5; TRAF2; EGFR; DHX9; STAU2; PRKRA; EIF2AK2; HECW2; TOP1; STAU1; DICER1; DHX30; EBNA1BP2; IGF2BP2; IGF2BP3; PABPC4; MOV10; ILF3; ILF2; HSP90AA1; EIF2A; NOC3L; GNL3; NOP2; HNRNPC; HIST1H1C; ELAVL1; NPM1; IL7R; gag; Jak2; TYK2; JAK1; EIF2S1;

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: EIF2AK2 antibody tested with Human Hela Cells (ARP36709_P050)