EGLN3 Antibody - C-terminal region (ARP52402_P050)

Catalog No: ARP52402_P050

WB Suggested Anti-EGLN3 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human brain

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EGLN3
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 77%; Zebrafish: 75%
• Peptide Sequence: Synthetic peptide located within the following region: FWSDRRNPHEVQPSYATRYAMTVWYFDAEERAEAKKKFRNLTRKTESALT
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-EGLN3 (ARP52402_P050) antibody is Catalog # AAP52402 (Previous Catalog # AAPP14346)

Target Info

• Reference: Ginouves,A., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (12), 4745-4750
• Gene Symbol: EGLN3
• Gene Full Name: Egl nine homolog 3 (C. elegans)
• Alias Symbols: PHD3, HIFPH3, HIFP4H3
• NCBI Gene Id: 112399
• Protein Name: Egl nine homolog 3
• Description of Target: EGLN3 catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. EGLN3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. It may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. EGLN3 promotes cell death through a caspase-dependent mechanism.
• Uniprot ID: Q9H6Z9
• Protein Accession #: NP_071356
• Nucleotide Accession #: NM_022073
• Protein Size (# AA): 239
• Molecular Weight: 27kDa
• Protein Interactions: FOXJ2; SERTAD1; NCAPH2; EFHC2; ABI2; SIAH2; REL; HIF1A; TTC23L; SQSTM1; HEXIM1; IKBKG; IKBKB; CHUK; MAPK7; MAPK6; MAPK1; PKM; EPAS1; UBD; MAGEA11; UBC; ATF4; WTIP; AJUBA; LIMD1; SPRY2; ADRB2; PRPF19; Myog; EGLN3; EGLN2; EGLN1; OS9; HIF3A; WDR83;