EGLN2 Antibody (OAGA06991)

Catalog No: OAGA06991

Size:100 ul

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using PHD1 antibody at 1:250 dilution.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PHD1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (red) at 1:2000.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PHD1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000, WB: 1:1000-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: EGLN2
• Gene Full Name: egl-9 family hypoxia inducible factor 2
• Alias Symbols: EIT6, PHD1, EIT-6, HPH-1, HPH-3, HIFPH1, HIF-PH1
• NCBI Gene Id: 112398
• Protein Name: egl nine homolog 2
• Description of Target: The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
• Uniprot ID: Q96KS0
• Protein Accession #: NP_444274.1
• Nucleotide Accession #: NM_053046.3