EGLN1 Antibody (OAGA08044)

Catalog No: OAGA08044

Size:100 ul

Untreated (–) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD2 antibody diluted at 1:2000.

PHD2 antibody detects PHD2 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PHD2 stained by PHD2 antibody diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI .

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-F, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PHD2. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.99 mg/ml
• Preservative: 0.025% ProClin 300
• Application Info: Suggested starting dilutions are as follows: IHC-Fr: 1:100-1:1000, WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Target Info

• Gene Symbol: EGLN1
• Gene Full Name: egl-9 family hypoxia inducible factor 1
• Alias Symbols: HPH2, PHD2, SM20, ECYT3, HALAH, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2
• NCBI Gene Id: 54583
• Protein Name: egl nine homolog 1
• Description of Target: The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).
• Uniprot ID: Q9GZT9
• Protein Accession #: NP_071334.1
• Nucleotide Accession #: NM_022051.2