EGLN1 Antibody (OAAJ06759)

Catalog No: OAAJ06759

Size:100 uL

Western blot analysis of extracts of various celllines, using EGLN1 antibody.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: Store at +4C. For long term storage, aliquot and store at -20C. Avoid freeze/thaw cycles.
• Immunogen: Recombinant proteinof human EGLN1
• Purification: Immunogen affinity chromatography
• Concentration: 1 mg/mL

Target Info

• Gene Symbol: EGLN1
• Alias Symbols: HPH2, PHD2, SM20, ECYT3, HALAH, HPH-2, HIFPH2, ZMYND6, C1orf12, HIF-PH2
• NCBI Gene Id: 54583
• Protein Name: Egl nine homolog 1
• Description of Target: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferentially recognized via a LXXLAP motif.
• Uniprot ID: Q9GZT9
• Protein Accession #: NP_071334
• Nucleotide Accession #: NM_022051
• Molecular Weight: 46 kDa