EGFBP2 ELISA Kit (Mouse) (OKCD00193)

Catalog No: OKCD00193 (Formerly GWB-KBBOC3)

Size:96WELLS

Mouse Egfbp2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	93-102%	90-101%	84-101%	78-104%
  EDTA plasma(n=5)	78-102%	93-102%	95-105%	90-104%
  heparin plasma(n=5)	90-101%	97-105%	84-105%	97-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kallikrein 13 (KLK13). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kallikrein 13 (KLK13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kallikrein 13 (KLK13), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kallikrein 13 (KLK13) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	90-98	95
  EDTA plasma(n=5)	80-93	80
  heparin plasma(n=5)	78-99	80

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kallikrein 13 (KLK13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kallikrein 13 (KLK13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Egfbp2 Microplate: 96 Wells (12 x 8 Well Strips)
  • Egfbp2 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Egfbp2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 6.2 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kallikrein 13 (KLK13). No significant cross-reactivity or interference between Kallikrein 13 (KLK13) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Egfbp2
• Gene Full Name: epidermal growth factor binding protein type B
• Alias Symbols: Egfbp-;EGF-BP B;Egfbp-2;epidermal growth factor-binding protein type B;glandular kallikrein K13;kallikrein 1b26;Klk1b26;mGk-13;PRECE-1;prorenin-converting enzyme 1;tissue kallikrein 13.
• NCBI Gene Id: 13647
• Protein Name: Epidermal growth factor-binding protein type B
• Description of Target: Cleaves REN2 at a dibasic site to yield mature renin.
• Uniprot ID: P36368
• Protein Accession #: NP_034245
• Nucleotide Accession #: NM_010115.6
• Protein Size (# AA): 261